How to Add Darkfield Capability to BH2 Microscopes
There are multiple ways to add darkfield to your BH2. These methods are described below
The simplest and least expensive method to add darkfield capabilities to a light microscope is to add a simple darkfield patch stop to the condenser, to block the central area of the aperture diaphragm from receiving light, thereby creating the hollow cone of illumination necessary for darkfield. In many scopes, the design of the condenser is such that a filter slot or carrier placed at the bottom of the condenser is physically close enough to the aperture diaphragm for a patch stop placed within the slot or carrier to provide effective darkfield for low-power objectives (4x – 20x). The design of the BH2 condensers complicate things a little bit, since the internal aperture diaphragm is too far removed from the bottom surface of the condenser mounting dovetail for a slot or carrier to be effective for darkfield. Fortunately, with the advent of 3D printing, it is a simple matter to fabricate an insert compatible with the BH2 condensers that, when pushed up into the bottom bore of the condenser, will position a patch stop in the appropriate location for effective darkfield.
Click here to see a PDF describing the BH2-DFR Darkfield / Rheinberg kit, made freely available by the Empire of Dirt Workshop. This kit is compatible with the BH2-CD Abbe condenser and the BH2-AAC aplanatic / achromatic condenser, and will provide darkfield or Rheinberg for 4x – 20x objectives.
Another method to add a simple darkfield patch stop to the BH2 is by using the BH2-PCD Zernike-style phase contrast condenser. This condenser contains an integral patch stop which is suitable for 4x – 20x objectives.
If darkfield is required for higher-power objectives, a dedicated darkfield condenser will likely be needed. Olympus made the BH2-DCD dry darkfield condenser, as well as the BH2-DCW oil-immersion darkfield condenser for the BH2 scopes. Both of these condensers are fairly rare, and each typically will fetch $400 or more when they do show up on ebay. The BH2-DCD is suitable for use with 4x – 40x objectives. This is a dry condenser and must never be used with oil immersion, or damage to the condenser will result. The BH2-DCW is an oil-immersion condenser suitable for use with 10x – 100x objectives. The BH2-DCW must always be oiled to the bottom surface of the slide, or no light will be visible through the eyepieces. To achieve darkfield using the BH2-DCW and a 100X objective, the objective must have an internal iris diaphragm to allow the NA to be reduced to below the upper NA of the condenser.
Many people have reported success using Amscope darkfield condensers on their BH2. […more information on this will be provided soon…]
